HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer)

For non-bovine milks, information regarding bioactive lipids is fragmented, unreliable or unavailable. The purpose of the current study was to analyse in milk dairy animals using modern analytical methods achieve most reliable results. Bioactive human were also analysed and used as a reference. A suite employed, namely High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS), Gas Chromatography (GC) Nuclear Magnetic Resonance (NMR). total lipid content determined, phospholipid, fatty acid, neutral glycosphingolipids ganglioside (GM3 GD3) levels measured. Lipid classes selected milks reliably characterised for first time, including gangliosides deer, camel sheep; cerebrosides buffalo; plasmalogens buffalo goat phospholipids deer. Our demonstrated advantage utilising range techniques order characterise diverse set lipids.